		Technical Data
C7920	Cre Recombinase

Description:
Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is a homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:500-1:5000
Western Blot (Native & recombinant protein): 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	2Q2151	Ascites

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Mouse

Concentration:

Not Determined

Immunogen:

Cre recombinase aa77-343 fusion protein.

Purity:

Ascites

Form

Supplied as a liquid. No preservatives added.

Specificity:

Recognizes both native and recombinant Cre recombinase at ~38kD from all species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hamilton, D.L. and Abremski, K., J. Mol. Biol. 178(2): 481-486 (1984). 2. Xin, H.B., et al., Physiol. Genomics 10: 210-215 (2002). 3. Pfeifer, A., et al., PNAS 98(20): 11,450-11,455 (2001).