Technical Data
C7920
Cre Recombinase
Description:
Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is a homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:500-1:5000
Western Blot (Native & recombinant protein): 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q2151Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not Determined
Immunogen:
Cre recombinase aa77-343 fusion protein (NM_004380).
Purity:
Ascites
Form
Supplied as a liquid. No preservatives added.
Specificity:
Recognizes both native and recombinant Cre recombinase at ~38kD from all species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hamilton, D.L. and Abremski, K., J. Mol. Biol. 178(2): 481-486 (1984). 2. Xin, H.B., et al., Physiol. Genomics 10: 210-215 (2002). 3. Pfeifer, A., et al., PNAS 98(20): 11,450-11,455 (2001).