Technical Data
C7944-74G
Crystallin, alpha (alpha Crystallin Protein, Alpha Crystallin, Alpha Crystallin Chain, CRYAB, Crystallin alpha 2, CRYA2, CTPP2, Heat Shock 20 kD Like Protein, Heat Shock Protein beta 5, HSPB5, NY-REN 27 Antigen, Renal Carcinoma Antigen NY-REN-27, Rosent
Description:
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its phosphorylated
state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:10000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH.
Purity:
Serum.
Form
Supplied as a liquid.
Specificity:
Recognizes human alpha crystallin. Species Crossreactivity: mouse, rat, bovine, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41:483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.
9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.