Technical Data
C7944-74L
Crystallin, alpha B
Description:
Alpha B-crystallin is a heat shock chaperone protein, up-regulated by stress. It has been shown to be expressed in the vertebrate eye lens and also found in cardiac and skeletal muscles, the kidney and the brain. Alpha B-crystallin is also found to be a component of Rosenthal fibers and a consistent tissue marker of epileptic foci.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detected alpha B-crystallin on 10ug of human heart tissue lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k11C236Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length recombinant protein corresponding to human alpha B-crystallin. Cellular Localization: Cytoplasm. Nucleus. Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing spec
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human alpha B-crystallin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.