Technical Data
C7944-75G
Crystallin, alpha B, phosphorylated (Ser59)
Description:
Alpha-crystallins composed of alpha-A (~19kD) and alpha-B (~19.2kD) subunits, are major water-soluble proteins accounting for almost 50% of the total protein of the mammalian transparent eye lens and they are also found in a variety of other tissues. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small heat shock proteins such as Hsp25. The alpha-crystallins and Hsp25 have been shown to act as molecular chaperones by suppression of heat-induced aggregation of other proteins and are efficient inhibitors of elastase. The alpha-B subunit like Hsp25 was also found to be a substrate for protein cross-linking by tissue-type transglutaminase. The alpha-crystallins may be involved in metabolic pathways important for the development, maintenance, or pathology of the lens. They prevent destabilized proteins from precipitating out of solution by forming a soluble high-molecular-weight (HMW) complex. The bovine HMW protein form of the lens is composed mostly of alpha-crystallin in a highly aggregated state which has markedly reduced chaperone ability compared to alpha-crystallin. NMR spectroscopy of the naturally occurring HMW complex shows that the short C-terminal extension of alpha-B subunit in HMW has lost its flexibility whereas the alpha-A subunit extension is still flexible. These results suggest that depletion of alpha- crystallin with age as it is converted to HMW, could be one of the contributing factors to cataract formation in the lens.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1:1000
Optimal dilutions to be determined by researcher.

Positive Control:
alpha Crystallin Protein

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa54-64 of human Crystallin, alpha B, phosphorylated at Ser59.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.0, 3% BSA, 0.1% sodium azide, 50% glycerol
Specificity:
Recognizes rat Crystallin, alpha B, phosphorylated (Ser59). Species Crossreactivity: mouse, bovine, porcine and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Augusteyn, R. C., Parkhill, E. M., and Stevens, A. (1992) Exp. Eye Res. 54: 219-228. 2. Narberhaus, F. (2002) Microbiol Mol Biol Rev 66(1): 64-93. 3. Merck, K. B., Groenen, P. J., Vooter, C. E., de Haard-Hoelman, W. A., Horwitz, J., Bloemendal, H. and de Jong, W. W. (1993) J. Biol. Chem. 268: 1046-1052. 4. MacRae, T.H. (2000) Cell Mol Life Sci 57(6): 899-913. 5. Head, M.W., Goldman, J.E. (2000) Neuropathol Appl Neurobiol 26(4): 304-312. 6. Armstrong, S.C., Shivell, C.L., Ganote, C.E., (2000) J Mol Cell Cardiol. 32(7):1301-1314. 7. Ito H, Iida K, Kamei K, Iwamoto I, Inaguma Y, Kato K.. (1999) FEBS Lett. 446(2-3): 269-272. 8. Kamei, A., Hamaguchi, T., Matsuura, N., Masuda, K. (2001) Biol Pharm Bull. 24(1): 96-99