Technical Data
C7944-75U
Crystallin, alpha B (Alpha-Crystallin B Chain, Alpha(B)-Crystallin, CRYAB, CRYA2, CTPP2, Heat Shock Protein beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27, Rosenthal Fiber Component)
Description:
Alpha B Crystallin is expressed in a number of normal tissues including muscle, neural and epithelial tissues and in various carcinomas such as renal cell carcinoma. The protein is also found in Lewy bodies and implicated in Lewy body dementia.

Applications:
Suitable for use in Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Immunohistochemistry: 1:80-1:160 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Brain
Immunohistochemistry (Paraffin Antigen unmasking) (Frozen Acetone fixation): Kidney Cytoplasmic staining pattern

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1G2JFSupernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide representing amino acids aa1-10 of human Alpha B Crystallin.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes human Alpha B Crystallin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leach I H, Tsang M L, Church R J, et al.. Alpha-B crystallin in the normal human myocardium and cardiac conducting system. Journal of Pathology. 173:255-260 (1994). 2. Pinder S E, Balsitis M, Ellis I O, et al.. The expression of alpha B-crystallin in epithelial tumours: a useful tumour marker? Journal of Pathology. 174:209-215 (1994). 3. Lowe J, McDermott H, Pike I, et al.. aB crystallin expression in non-lenticular tissues and selective presence in ubiquitinated inclusion bodies in human disease. Journal of Pathology. 166: 61-68 (1992). 4. Iwaki T, Jume-Iwaki A and Goldman J E. Cellular distribution of alpha B crystallin in non-lenticular tissues. Journal of Histochemistry and Cytochemistry. 38:31-39 (1990). 5. Lowe J, Landon M, Pike I, et al.. Dementia with a-amyloid deposition: involvement of aB crystallin supports two main diseases. Lancet. 336: 515-516 (1990).