		Technical Data
C7949-01G	Cullin 1 (CUL-1)

Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 1 (CUL 1) is an essential component of SCF E3 Ubiquitin ligase complex which mediates the ubiquitation of proteins involved in cell cycle progression, signal transduction and transcription.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide (DGEKDTYSYLA) corresponding to aa742-752 of human Cullin 1

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes human CUL 1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Michel, J.J. & Xiong, Y. (1998) Cell Growth Differ. 9,435-449. Zheng, N. et al. (2002) Nature 416, 703-709. Jackson, P.K. & Eldridge, A.G. (2002) Mol. Cell. 9,923-925.