Technical Data
C7949-01G
Cullin 1 (CUL-1)
Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 1 (CUL 1) is an essential component of SCF E3 Ubiquitin ligase complex which mediates the ubiquitation of proteins involved in cell cycle progression, signal transduction and transcription.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide (DGEKDTYSYLA) corresponding to aa742-752 of human Cullin 1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human CUL 1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Michel, J.J. & Xiong, Y. (1998) Cell Growth Differ. 9,435-449. Zheng, N. et al. (2002) Nature 416, 703-709. Jackson, P.K. & Eldridge, A.G. (2002) Mol. Cell. 9,923-925.