Technical Data
C7949-13
Cullin 2 (CUL-2)
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Technical Data |
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C7949-13 |
Cullin 2 (CUL-2) |
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Description: In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2). A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identi ed (cul1, 2, 3, 4a, 4b, and 5) (1) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) . The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Immunogen): 0.1-1.0ug/ml Western Blot: 1-3ug/ml Immunoprecipitation (Native): 5-10ug Optimal dilutions to be determined by the researcher. Positive Control: CUL2 recombinant protein and cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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