Technical Data
Cullin 2 (CUL-2)
In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2). A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identi ed (cul1, 2, 3, 4a, 4b, and 5) (1) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) . The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A.

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1.0ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 5-10ug
Optimal dilutions to be determined by the researcher.

Positive Control:
CUL2 recombinant protein and cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide derived from the C-terminus of the human CUL2.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Recognizes human CUL2 protein at ~83kD. Does not crossreact with the related CUL1 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kipreos, E.T., et al; Cell 85:829839 (1996). 2. Pause, et al, Proc. Natl. Acad. Sci. U.S.A., 94:2156:2161 (1997). 3. Pagano, M., FASEB J. 11:10671075 (1997).