		Technical Data
C7949-20F	Cullin 3 (NT) (CUL-3)

Description:
CUL-3 is a member of the family of human cullin genes (CUL-1,-2,-3,-4a,-4b, and-5), homologous to the S. cerevisiae cdc53 gene. Cullin-3 is proposed to be a part of E3 protein-ubiqutinnn ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase. Cul-3-/-mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide, MSNLSKGTGSRK, corresponding to aa1-12 of human Cul3, N-terminus (KLH).

Purity:

Serum

Form

Supplied as a liquid, 0.01% sodium azide.

Specificity:

Recognizes human Cullin 3 at ~88.9kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Furukawa, M., et al., (2003), "Targeting of ubiquitination by BTB-Cullin3-Roc1 ubiquitin ligases", Nat. Cell Biol., 5, 1001-1007. 2. Kipreos, E. T., et al., (1996), "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family", Cell, 85 (6), 829-839. 3. Michel, J.J., et al., (1998), "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A", Cell Growth Differ., 9 (6), 435-449. 4. Ohta, T., et al., (1999), "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity", Mol. Cell., 3 (4), 535-541.5. Mathias, N., et al., (1996), "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins", Mol. Cell Biol., 16:12, 6634-6643.