Technical Data
C7949-21B
Cullin 3 (CUL-3)
Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 3 (CUL 3) is involved in the ubiquitination of cyclin E forming a tight complex with this protein which leads to its degradation by proteosomes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide (MSNLSKGTGSRK) corresponding to aa1-12 of human Cullin 3
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human CUL 3
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Du, M. et al. (1998) J. Biol. Chem. 273,24289-24292. Singer, J.D. et al. (1999) Genes & Dev. 13, 2375-2387. Van den Heuvel, S. (2004) Curr. Biol. 14, R59-61. Sumara, I. et al. (2007) Dev. Cell. 12, 887-900.