Technical Data
C7949-21H1
Cullin 4 (CUL-4A)
Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF. It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Western Blot: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to aa1-20 of human Cullin 4a
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human CUL 4a
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Chen, L-C. et al. (1998) Cancer Res. 58,3677-3683. Dai, Q. & Wang, H. (2006) Cell Div. 1,14. Higa, L.A et al. (2006) Cell Cycle 5,1675-1680. Angers, S. et al. (2006) Nature 443,590-593.