		Technical Data
C7949-21H1	Cullin 4 (CUL-4A)

Description:
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF. It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Western Blot: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to aa1-20 of human Cullin 4a

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes human CUL 4a

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Chen, L-C. et al. (1998) Cancer Res. 58,3677-3683. Dai, Q. & Wang, H. (2006) Cell Div. 1,14. Higa, L.A et al. (2006) Cell Cycle 5,1675-1680. Angers, S. et al. (2006) Nature 443,590-593.