Technical Data
C8349
CXCR3 (C-X-C Chemokine Receptor Type 3, CXC-R3, CXCR-3, CKR-L2, G-Protein-coupled Receptor 9, Interferon-inducible Protein 10 Receptor, IP-10 Receptor, CD183, GPR9)
Description:
CXCR3 is a G protein­coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP­10 (CXCL10), and I­TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Applications:
Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Neutralization (ND50): ~0.3-1.5ug/ml in the presence of 7ng/ml of recombinant human CXCL11/l-TAC, using the human CXCR3 transfected BaF/3 cells in a chemotaxis assay.
The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface.
Flow Cytometry: 2.5x10e6 cells using whole human blood lymphocytes.
Immunohistochemistry: 8-25ug/ml detected CXCR3 in immersion fixed paraffin-embedded sections of human tonsil.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo

Hybridoma:
Mouse myeloma with B cells obtained from a mouse inoculated with human CXCR3 transfected NS0 mouse myeloma cells.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Ar1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa1-368 of human CXRR3 expressed in NS0 cells (P49682).
Purity:
Purified by Protein G affinity chromatography. Endotoxin: 0.1EU/ug (LAL).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul of sterile PBS.
Specificity:
Recognizes human CXCR3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.