Technical Data
C8452-04C
Cyclin A
Description:
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:100. Incubate for 2hrs at RT
Immunoprecipitation: Native verified
Immunohistochemistry (Formalin-fixed only): 1:40 for 60 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.

Positive Control:
HeLa cells,Tonsil.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k5G86Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant N-terminal fragment of human cyclin A protein. Epitope: N-terminal. Cellular Localization: Nuclear.
Purity:
Culture supernatant
Form
Supplied as a liquid with 0.09% sodium azide.
Specificity:
Recognizes human Cyclin A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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