		Technical Data
C8452-04C	Cyclin A

Description:
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:100. Incubate for 2hrs at RT
Immunoprecipitation: Native verified
Immunohistochemistry (Formalin-fixed only): 1:40 for 60 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.

Positive Control:
HeLa cells,Tonsil.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	5G86	Supernatant

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant N-terminal fragment of human cyclin A protein. Epitope: N-terminal. Cellular Localization: Nuclear.

Purity:

Culture supernatant

Form

Supplied as a liquid with 0.09% sodium azide.

Specificity:

Recognizes human Cyclin A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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