Technical Data
C8452-06B
Cyclin A
Description:
Cyclins controls the transitions between the phases of the eukaryotic cell cycle as regulatory subunits of the cyclin dependentkinases (cdk). Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Cyclin A first identified in sea urchin embryos is a 33kD protein identical to adenovirus e1a-associated protein p60. It can bind and activate the kinases p33cdk2 and p34cdc2. In addition to its mitotic functions, cyclin A is essential for progression through S phase. During S phase, cyclin A forms complex containing cdk2, the pRB-related protein p107, and the transcription factor E2F. It may indeed be directly involved in DNA replication; it colocalizes with the DNA replication complex and was found in a cellular activity that promotes large tumor-antigen-dependent simian virus 40DNA replication in Gl-phase cell extracts. It maps to 4q25-q31 region of human chromosome.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:500
Immunohistochemistry (formalin fixed paraffin embedded): 1:20-1:40
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lung cell line

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E131Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to the N-terminal fragment of cyclin A protein. Cellular Localization: Nuclear.
Purity:
Supernatant
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human Cyclin A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bodey et al. Modern Pathol 7: 846, 1994. 2. Inohara et al. Arch Dermatol Res 286: 504, 1994. 3. Dutta et al. Natl Acad Sci 92: 5386, 1995. 4. Berthold et al. Cell Biology 91: 5490, 1994.