Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
MW of Antigen: 60kD
Applications and Recommended Dilutions:
Western Blotting (Ab 5-10ug/ml for 2hrs at RT)
Immunoprecipitation (Denatured verified) (Use Protein A) (Ab 10ug/mg protein lysate)
Immunohistochemistry (Formalin/paraffin) ( Ab-6 is better) (Ab 5-10ug/ml for 30 min at RT) ** [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control: HeLa cells. Tonsil.
Cellular Localization: Nuclear
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|A synthetic peptide from the C-terminusl of human cyclin A.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8452-17X|
|Recognizes Cyclin A. Species Crossreactivity: human, mouse, rat.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Dutta A; et al. Proc Natl Acad Sci USA, 1995, 92(12):538690. 2. Gong J; et al. Leukemia, 1995, 9(5):8939.|
Additional: 1. Gong J; Traganos F; Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Research, 1995, 220(1):22631. 2. Gong J; Traganos F; Darzynkiewicz Z. Growth imbalance and altered expression of cyclins B1, A, E, and D3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth and Differentiation, 1995, 6(11):148593. 3. Jeffrey PD; Russo AA; Polyak K; Gibbs E; Hurwitz J; Massague J; Pavletich NP. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex [see comments]. Nature, 1995, 376(6538):31320. 4. Bodey B; Williams RT; Carbonaro-Hall DA; Horvath A; Tolo VT; Luck JV Jr; Taylor CR; Hall FL. Immunocytochemical detection of cyclin A and cyclin D in formalin-fixed, paraffin-embedded tissues: novel, pertinent markers of cell proliferation. Modern Pathology, 1994, 7(8):84652. 5. Inohara S; Kitano Y. Immunohistochemical detection of cyclin D and cyclin A in human hyperproliferative epidermis. Archives for Dermatological Research. Archiv fur Dermatologische Forschung, 1994, 286(8):504- 6. 6. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):65517. 7. Faha B; Harlow E; Lees E. The adenovirus E1A-associated kinase consists of cyclin Ep33cdk2 and cyclin A-p33cdk2. J of Virol, 1993, 67:245665. 8. Lees EM; Harlow E. Sequences within the conserved cyclin box of human cyclin A are sufficient for binding to and activation of cdc2 kinase. Mol and Cell Biol, 1993, 13(2):1194- 201. 9. Tommasino M; Adamczewski JP; Carlotti F; Barth CF; Manetti R; Contorni M; Cavalieri F; Hunt T; Crawford L. HPV16 E7 protein associates with the protein kinase p33CDK2 and cyclin A. Oncogene, 1993, 8(1):195202. 10. Woodman PG; Adamczewski JP; Hunt T; Warren G. In vitro fusion of endocytic vesicles is inhibited by cyclin A-cdc2 kinase. Molecular Biology of the Cell, 1993, 4(5):54153. 11. Ewen ME; Faha B; Harlow E; Livingston DM. Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins. Science, 1992, 255(5040):857. 12. Faha B; Ewen ME; Tsai LH; Livingston DM; Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science, 1992, 255(5040):8790. 13. Kobayashi H; Stewart E; Poon R; Adamczewski JP; Gannon J; Hunt T. Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Molecular Biology of the Cell, 1992, 3(11):127994. 14. Lees E; Faha B; Dulic V; Reed SI; Harlow E. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner. Genes and Development, 1992 Oct, 6(10):187485. 15. Williams RT; Carbonaro-Hall DA; Hall FL. Copurification of p34cdc2/p58cyclin A prolinedirected protein kinase and the retinoblastoma tumor susceptibility gene product: interaction of an oncogenic serine/threonine protein kinase with a tumor-suppressor protein. Oncogene, 1992, 7:42332.