Technical Data
C8453-17
Cyclin B1
Description:
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.

MW of Antigen: 62kD

Epitope: aa1-306

Applications and Recommended Dilutions:
Immunofluorescence
Immunoprecipitation (Native and denatured) (Use Protein A; Ab 2ug/mg protein lysate)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Positive Control: LS174T cells. Tonsil or breast carcinoma

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3F136Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human cyclin B1 protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8453-17X
Specificity:
Excellent for staining of formalin/paraffin tissues. Species Crossreactivity: human, mouse, rat hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kung AL, et. al. J Biol Chem, 1993;268:1–9. 2. Faha B, et. al. J Virology, 1993;67:2456–2465. 3. Zhang H, et. al. Mol Biol Cell, 1993;4:897–906. 4. Coleman TR, et. al. Cell, 1993;72:919–929.