Technical Data
CDKN1b, ID (Cyclin-dependent Kinase Inhibitor 1b (P27, kip1), P27-like Cyclin-dependent Kinase Inhibitor, CDKN1B (1 of 3), Dr.75267)
Cell proliferation is tightly controlled by cyclindependent kinases (CDKs) that function sequentially during the cell cycle. These kinases phosphorylate and thereby regulate key substrates involved in cell cycle progression. The activity regulation of CDKs involve the interaction with additional proteins and post- translational modifications. Essential positive regulators of CDKs are cyclins, the regulatory subunits of cyclin/CDK kinase complexes that are expressed periodically during the cell cycle. Furthermore, CDKs are controlled by phosphorylation that either stimulates or represses catalytic activity. Finally, CDK inhibitors (CKIs) have been shown to interact with distinct cyclin/CDK complexes, thereby interfering with their catalytic activity. p27KIP1 (p27) is a CKI that was originally identified as a protein capable of inhibiting G1 cyclin/CDK complexes. p27 was discovered as a protein whose expression is induced by different growth inhibitory agents, including tumor growth factor beta. Thus, p27 links proliferative and anti-proliferative signals and controls the transition from the G1 into the S phase of the cell cycle.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.

Control Peptide: C8471-63Q

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
250ul-20CBlue IceZebrafishRabbit
Not determined
Synthetic peptide corresponding to the C-terminal region of zebrafish CDKN-1b.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.1% sodium azide, 50% glycerol.
Recognizes zebrafish CDKN-1b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nurse, P. (2000) Cell 100, 71-8. 2. Kastan, M. B. and Bartek, J. (2004) Nature 432, 316-23. 3. Malumbres, M. and Barbacid, M. (2001) Cancer 1, 222-31. 4. Obaya, A. J. and Sedivy, J.M. (2002) Cell. Mol. Life Sci. 59, 126-42. 5. Sherr, C. J. and Roberts, J. M. (1999) Genes Dev. 13, 1501-12. 6. Polyak, K., et al. (1994) Genes Dev. 8, 9-22. 7. Polyak, K., et al. (1994) Cell 78, 59-66. 8. Slingerland, J. M., et al. (1994) Mol. Cell. Biol. 14, 3683-94. 9. Toyoshima, H. and Hunter, T. (1994) Cell 78, 67-74.