Technical Data
C8850-05A
Cyclophilin A (Peptidyl-prolyl Cis-trans Isomerase A, PPIase A, Cyclosporin A-binding Protein, Rotamase A, PPIA, CYPA)
Description:
Cyclophilin A, also called Peptidylprolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis-and trans-proline residues critical for proper folding of proteins. Cyclophilin is also incorporated into many viruses, including HIV1, where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin, making it a useful inhibitor for studying calcineurin activity.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.1mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa1-185 from human Cyclophilin A expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity:
Recognizes human Cyclophilin A. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119. 2. Cantin, R. et al. (2005) J. Virology 79:6577. 3. Liu, J. et al. (1992) Biochemistry 31:3896. 4. Wiegers K. and H.G. Krausslich (2002) Virology 294:289. 5. Liu, J. et al. (1991) Cell 66:807.