Technical Data
Cyclophilin B (PPIB, Peptidylpropyl Isomerase B)
Cyclophilin B is a 24kD glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25aa signal sequence plus a 191aa mature region. There is a partial heparin-binding sequence (aa27-34), a PPIase domain (aa47-204) and a C-terminal ER retention motif (aa213-216).

Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml detects Cyclophilin B in HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line and NRK rat normal kidney cells.
Immunocytochemistry: 8-25ug/ml. Immersion fixed HeLa human cervical epithelial carcinoma cell line
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a9H108Affinity Purified
100ug-20CBlue IceHumanMouse
Recombinant protein corresponding to Asp34-Glu216 from human Cyclophilin B expressed in E. coli (Accession #P23284). Species Sequence Homology: mouse (95%).
Purified by Protein A affinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human Cyclophilin B. Does not crossreact with recombinant human Cyclophilin A. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.