Technical Data
Cysteine-rich, Angiogenic Inducer 61 (CYR61) Recombinant, Human
Molecular Biology Storage: -20CShipping: Blue Ice
CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.5kD protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK

Biological Activity:
Determined by the dose dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0ug/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile ddH2O and a carrier protein. Aliquot and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
Source: E. coli
Purity: 95% by SDS-PAGE and HPLC analyses. Endotoxin: 1ng/ug.
Form: Supplied as a lyophilized powder from 10mM sodium citrate, 50mM sodium chloride, pH 3.0. Reconstitute with sterile ddH2O and a carrier protein to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.