Technical Data
C9095-11J
Cytochrome P450 1A1 cDNA Probe, Human, BioGenomics™
1ug
Molecular Biology Storage: -70°CShipping: Dry Ice
Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs). Both CYPs and FMOs are localized in the endoplasmic reticulum. Collectively, these enzymes catalyze the oxidation of a very broad range of substrates. Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity.

Size of the cDNA:
1.5Kb

Applications:
Suitable for use for the synthesis, by standard random priming or nick translation techniques, of radiolabeled probes for investigations of P450 1A2 gene expression. Other applications not tested.

Optimum dilutions to be determined by researcher.

Storage and Stability:
Can be stored for at least one year at -70°C. Avoid repeated freeze/thaw cycles.
Concentration: 0.1ug/ul
Form: Supplied in TE buffer.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Nebert, D.W., and Gonzalez, F.J., Ann. Rev. Biochem., 56 : 945 (1987)2. Guengerich, F.P., Cancer Research, 48 : 2946 (1988)3. Nebert, D.W., et al., DNA, 8 : 1 (1989)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.