Technical Data
CYP1B1 (Cytochrome P450 1B1, CYPIB1)
CYP1B1 is a 543aa intracellular protein localized to the endoplasmic reticulum. It belongs to the cytochrome P450 super family of enzymes which are monooxygenases and it catalyzes many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol and is also involved in the metabolism of substances active in the eye growth and differentiation and hence plays a putative role in ocular development. Mutated proteins are known to cause primary congenital glaucoma.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human brain lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa200-250 of human CYP1B1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human CYP1B1. Species Crossreactivity: chimpanzee, monkey. Species sequence homology: mouse, rat, equine, canine, bovine, zebrafish, opossum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hanna, IH. et al. Cancer. Res.. 60:3440-3444 (2000). 2. Vincent, AL. et al. Am. J. Hum. Genet. 70:448-460 (2002). 3. Stoilov, I. et al. Am. J. Hum. Genet. 62:573-584 (1998).