Technical Data
Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily.Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti-Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanMouse
Not determined
Prepared from a mixture of human cytokeratins.
15mM Sodium Azide; Whole Serum, 7% Horse serum, 10ug/ml Phenol red
Recognizes human cytokeratins 1, 4, 5, 6, 8, 10, 13, 18, and 19 in immunoblotting. It is a broad spectrum reagent which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudo-stratified epithelia. It does not react with non-epithelial normal human tissues. Species Crossreactivity: The Anti-Pan Cytokeratin (mixture) exhibits a wide interspecies cross reactivity down to Xenopus laevis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lane EB & Alexander CM (1990) Semin Cancer Biol 1:165-79. 2. Moll R et al. (1982) Cell 31:11-24.