Technical Data
C9097-04
Cytokeratin 1,10
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin fixed sections.
Note: Pretreatment with type XXIV protease (0.5mg/ml) produces best results. HIER (Microwave citrate buffer pH 6.0) produces some staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1048Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Keratin 1 and 10
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide
Specificity:
Recognizes the suprabasal cells of human stratified squamous epithelia. Species Crossreactivity: mouse and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brockaert, D., et al., Arch. Dematol. Res. 282: 383-91 (1990). 2. Markey, A., et al., J. of Invest. Dermatol. 97: 763-70 (1991).