Technical Data
Cytokeratin 10
AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulfide linked precursor that is cleaved to release the mature 30kD homodimer.

Suitable for use in Immunohistochemistry (frozen, paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50, requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.

Spleen cells from immunized mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.

Recommended Positive Control Tissue:
Human normal skin

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Recommended Negative Controls:
Mouse IgG1 for use in human studies.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul-20CBlue IceHumanMouse
Not determined
Cytoskeletal extract from epidermis of normal human skin.
Tissue Culture Supernatant
Supplied as a liquid, .0.09% sodium azide.
Recognizes the 56kD cytokeratin 10, found in a variable number of cells in the suprabasal layer of stratified epithelia, and in more differentiated areas of some squamous carcinomas. Species Crossreactivity: Human, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leigh, I.M. et al. (1993). Monospecific monoclonal antibodies to keratin I carboxy terminal (sythetic peptide) and to keratin 10 as markers of epidermal differentiation. Br. J. Dermatol. 129: 110 - 119.