Technical Data
C9097-09D
Cytokeratin 10
Description:
AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulfide linked precursor that is cleaved to release the mature 30kD homodimer.

Applications:
Suitable for use in Immunohistochemistry (frozen, paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50, requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.

Hybridoma:
Spleen cells from immunized mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.

Recommended Positive Control Tissue:
Human normal skin

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Recommended Negative Controls:
Mouse IgG1 for use in human studies.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A400Supernatant
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Cytoskeletal extract from epidermis of normal human skin.
Purity:
Tissue Culture Supernatant
Form
Supplied as a liquid, .0.09% sodium azide.
Specificity:
Recognizes the 56kD cytokeratin 10, found in a variable number of cells in the suprabasal layer of stratified epithelia, and in more differentiated areas of some squamous carcinomas. Species Crossreactivity: Human, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leigh, I.M. et al. (1993). Monospecific monoclonal antibodies to keratin I carboxy terminal (sythetic peptide) and to keratin 10 as markers of epidermal differentiation. Br. J. Dermatol. 129: 110 - 119.