Technical Data
C9097-11D
Cytokeratin 10
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Applications:
Suitable for use in Immunohistochemistry, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100
Immunohistochemistry (frozen and paraffin sections): 1:50-1:100 using ABC detection reagent.
Western Blot: 1:50-1:500
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k4A27Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Cytoskeletal preparation extracted from human epidermis.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity:
Recognizes exclusively human cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. Species crossreactivity: feline and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smedts F, et al., Am. J. Path. 136: 657-668 (1990). 2. Ramaekers F, et al. Exp. Cell Res. 170: 235-249 (1987). 3. Smedts F, et al. Am. J. Path. 141: 497-511 (1992). 4. Raats JMH, et al. J. Cell Biol. 111: 1971-1985 (1990). 5. Ramaekers FCS, et al. Am. J. Pathol. 136: 641-655 (1990). 6. Puts JJG, et al. Int. J. Gynecol. Pathol. 4: 300-313 (1985). 7. Wetzels RHW,et al. Am. J. Path. 138: 751-763 (1991). 8. Wetzels RHW, et al. Histopathol. 20: 295-303 (1992).  9. Ramaekers F, et al. Lab. Invest. 49: 353-361 (1983). 10. Schaafsma HE,et al.Am. J. Path. 136: 329-343 (1990).