Technical Data
C9097-12B
Cytokeratin 13
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Applications:
Suitable for use in Immunohistochemistry (frozen sections) and Western Blotting. Other applications not tested.

Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a4A29Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Cytokeratin 13
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Reacts with non-cornified squamous epithelia, except cornea. Species crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smedts F, et al., Am. J. Path. 136: 657-668 (1990). 2. Ramaekers F, et al. Exp. Cell Res. 170: 235-249 (1987). 3. Smedts F, et al. Am. J. Path. 141: 497-511 (1992). 4. Raats JMH, et al. J. Cell Biol. 111: 1971-1985 (1990). 5. Ramaekers FCS, et al. Am. J. Pathol. 136: 641-655 (1990). 6. Puts JJG, et al. Int. J. Gynecol. Pathol. 4: 300-313 (1985). 7. Wetzels RHW,et al. Am. J. Path. 138: 751-763 (1991). 8. Wetzels RHW, et al. Histopathol. 20: 295-303 (1992).  9. Ramaekers F, et al. Lab. Invest. 49: 353-361 (1983). 10. Schaafsma HE,et al.Am. J. Path. 136: 329-343 (1990).