Technical Data
C9097-44S
Cytokeratin 8,18
Description:
Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.

Applications:
Suitable for use in Immunohistochemistry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: Detects a band at ~52.2kD (originally reported also as 45 kD cytokeratin 18 in the Moll-catalogue).
Immunohistochemistry: 1:5-1:10 frozen and paraffin-embedded tissue require Proteolytic treatment with pepsin or pronase.
Immunocytochemistry: Cell Supensions require Proteolytic treatment with pepsin or pronase.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a8J254Ascites
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Isolated from human breast cancer carcinoma cell line MCF-7.
Purity:
Ascites
Form
Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes all human simple epithelia including glandular epithelia. Does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. Epitope has been mapped to aa353-367 on alpha helical rod domain of cytokeratin 8. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.