Description:
Cytokeratin Type II constitutes the Type II intermediate filaments (IFs) of the intracytoplasmatic cytoskeleton, which is present in all mammalian epithelial cells. The type 2 cytokeratins consist of basic or neutral, high molecular weight proteins which are arranged in pairs of heterotypic Type I and Type II keratin chains in vivo, coexpressed during differentiation of simple and stratified epithelial tissues.
Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Cell sorting and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (frozen tissue and cytological material): 10-20ug/ml; dilute with PBS, pH 7.4; Incubation Time: 1 hr at RT.
Optimal dilutions to be determined by the researcher.
Biotin Coupling Ratio:
5mol Biotin/1mol IgG.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 5F224 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 250ul | | -20°C | Blue Ice | Human | Mouse |
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| Concentration: |
| Not determined. |
| Immunogen: |
| Cytoskeletal proteins from cultured human MCF-7 cells. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid containing 0.09% sodium azide. labeled with Biotin. |
| Specificity: |
| Recognizes human Cytokeratin Type II. Species Crossreactivity: bovine, amphibia. Reactivities on Cultured Cells: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12 bovine BMGE+H, BMGE-H, MDBK. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Achtstätter, Th., et al., Cytokeratin filaments and desmosomes in the epithelial cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129-149 (1989). 2. Gould, V.E., et al., Coexpression patterns of vimentin and glial filament protein with cytokeratin in the normal, hyperplastic and neoplastic breast. Am. J. Pathol. 137, 1143-1155 (1989). 3. Heid, et al., Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37, 137-157 (1988). 4. Moll, R., et al., The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982). 5. Moll, R., et al., Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111, 567-580 (1990).
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