Cytokeratin Type II constitutes the Type II intermediate filaments (IFs) of the intracytoplasmatic cytoskeleton, which is present in all mammalian epithelial cells. The type 2 cytokeratins consist of basic or neutral, high molecular weight proteins which are arranged in pairs of heterotypic Type I and Type II keratin chains in vivo, coexpressed during differentiation of simple and stratified epithelial tissues.
Suitable for use in Flow Cytometry, ELISA, Western Blot, Cell sorting and Immunohistochemistry. Other applications not tested.
Immunohistochemistry (frozen tissue and cytological material): 10-20ug/ml; dilute with PBS, pH 7.4; Incubation Time: 1 hr at RT.
Optimal dilutions to be determined by the researcher.
Biotin Coupling Ratio:
5mol Biotin/1mol IgG.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not determined.|
|Cytoskeletal proteins from cultured human MCF-7 cells.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid containing 0.09% sodium azide. labeled with Biotin.|
|Recognizes human Cytokeratin Type II. Species Crossreactivity: bovine, amphibia. Reactivities on Cultured Cells: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12 bovine BMGE+H, BMGE-H, MDBK.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Achtstätter, Th., et al., Cytokeratin filaments and desmosomes in the epithelial cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation 40, 129-149 (1989). 2. Gould, V.E., et al., Coexpression patterns of vimentin and glial filament protein with cytokeratin in the normal, hyperplastic and neoplastic breast. Am. J. Pathol. 137, 1143-1155 (1989). 3. Heid, et al., Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation 37, 137-157 (1988). 4. Moll, R., et al., The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982). 5. Moll, R., et al., Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111, 567-580 (1990).|