Technical Data
C9100-21L
Cytomegalovirus Glycoprotein B, Late Cytoplasmic Antigen (CMV gB, CMV Glycoprotein GP55, HCMV, Human Herpes virus 5, HHV-5, HHV5, UL55)
Description:
Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human Herpes virus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of ~100kD.

Applications:
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry (frozen sections). Other applications not tested.

Recommended Dilution:
Immunofluorescence: 0.02-1ug/slide; Reactive only in acetone fixed CMV infected cells or frozen sections.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-14C: IgG, Fc (HRP) Pab Gt x Mo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10B2663Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Native
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes cytomegalovirus glycoprotein B, a viral ligand for CD209 which allows capture of viral particles by dendritic cells. Does not react with HSV, VSV or EBV.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.