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Technical Data |
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C9100-21L |
Cytomegalovirus Glycoprotein B, Late Cytoplasmic Antigen (CMV gB, CMV Glycoprotein GP55, HCMV, Human Herpesvirus 5, HHV-5, HHV5, UL55) |
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Description: Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of ~100kD. Applications: Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry (frozen sections). Other applications not tested. Recommended Dilution: Immunofluorescence: 0.02-1ug/slide; Reactive only in acetone fixed CMV infected cells or frozen sections. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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