Technical Data
CS005-005
Custom Monoclonal Antibodies (Standard Protocol, 120 Days)
per Mab
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Technical Data |
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CS005-005 |
Custom Monoclonal Antibodies (Standard Protocol, 120 Days) |
per Mab |
| Custom Services | Storage: -20°CShipping: Blue Ice |
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Monoclonal Antibody Production Total Time: ~4 months. * Free peptide designing * Immunization of 5 Balb/c mice * Fusion of spleen cells with SP2/0 myeloma cells. * ELISA screening for up to 3,000 clones. * Free Western blot screening for protein antigen * Guaranteed IgG isotype * Progress Tracking * ELISA positive guaranteed or your money back. Phase I *Immunization and boosts of 5 Balb/c mice with standard immunization protocol. *ELISA evaluation of titer prior to selection for fusion. *Approximately 3-4mg protein immunogen or 2mg conjugated peptide immunogen and 0.5mg free peptide are required. Phase II *Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells. *Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation. Phase III *2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped and expanded. Additional Services: Peptide synthesis and conjugation Peptide synthesis without conjugation Peptide conjugation Additional clones (>10) in Phase II Negative screening Extra screening with IHC Additional clones (>2) in Phase III Cell-line adaptation to serum free medium Cell banking and store in 10 ampules after first two months Ascites production Antibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum) Antibody culture in bioreactor Antibody culture in flask Antibody purification by protein G from one liter of medium Antibody purification by protein G from ascites (20mg) Antibody epitope mapping by peptide library Antibody conjugation with FITC Antibody conjugation with HRP Antibody biotinylation Client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality. |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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