Custom Monoclonal Antibody Production
Monoclonal Antibody Production
* Free peptide designing
* Immunization of 5 Balb/c mice
* Fusion of spleen cells with SP2/0 myeloma cells.
* ELISA screening for up to 3,000 clones.
* Free Western blot screening for protein antigen
* Guaranteed IgG isotype
* Progress Tracking
* ELISA positive guaranteed
*Immunization and boosts of 5 Balb/c mice with standard immunization protocol.
*ELISA evaluation of titer prior to selection for fusion.
*Approximately 3-4mg protein immunogen or 2mg conjugated peptide immunogen and 0.5mg free peptide are required.
*Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells.
*Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation.
*2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped and expanded.
Peptide synthesis and conjugation
Peptide synthesis without conjugation
Additional clones (>10) in Phase II
Extra screening with IHC
Additional clones (>2) in Phase III
Cell-line adaptation to serum free medium
Cell banking and store in 10 ampules after first two months
Antibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum)
Antibody culture in bioreactor
Antibody culture in flask
Antibody purification by protein G from one liter of medium
Antibody purification by protein G from ascites (20mg)
Antibody epitope mapping by peptide library
Antibody conjugation with FITC, HRP, or Biotin
Client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.