Technical Data
D1200-01A
DCC (Netrin Receptor DCC, Colorectal Cancer Suppressor, Immunoglobulin Superfamily DCC Subclass Member 1, Tumor Suppressor Protein DCC, IGDCC1)
Description:
Deleted in colorectal cancer (DCC) is a 170-190kD transmembrane glycoprotein that contains four Ig-like domains and six fibronectin type III repeats in its extracellular domain. DCC is expressed on axons during development where its binding to Netrin-1 regulates axon migration and myelination. It inhibits apoptosis when bound by Netrin-1 and can induce apoptosis in the absence of ligand. DCC also functions as an adhesion molecule and a tumor suppressor on intestinal epithelial cells. Deficiencies in DCC function are associated with the development and metastasis of many tumors. Within aa1323-1447 of the cytoplasmic domain, human DCC shares 98% aa sequence identity with the mouse and rat DCC.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human DCC.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2B10K82Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified, E. coli-derived, recombinant human DCC (aa1323-1447; Accession #P43146).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.2um filtered solution in PBS.
Specificity:
Recognizes human DCC. Species sequence homology: mouse, rat (98%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.