Technical Data
D1560-01C1
DDAH2 (Dimethylarginine dimethylaminohydrolase II, NG30, G6a, OTTHUMP00000174406, OTTHUMP00000029307, DADB-110M10.5, OTTHUMP00000062666, NG-dimethylarginine dimethylamino hydrolase homolog, Dimethylarginine dimethylaminohydrolase II, DDAHII, NG30, G6a, DD
Description:
DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to SSLCLVLSTRPHS of human DDAH2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human DDAH2. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pasini AF, et al. Nebivolol treatment reduces serum levels of asymmetric dimethylarginine and improves endothelial dysfunction in essential hypertensive patients. Am J Hypertens. 2008 Nov;21(11):1251-7. PMID: 18772860 2. Imamura M, et al. Alterations of NOS, arginase, and DDAH protein expression in rabbit cavernous tissue after administration of cigarette smoke extract. Am J Physiol Regul Integr Comp Physiol. 2007 Nov; 293(5): R2081-9. PMID: 17881617 3. Hasegawa K, et al. Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2.Circ Res. 2007 Jul 20; 101 (2): e2-10. PMID: 17601800 4. Wakino S, et al. Pioglitazone lowers systemic asymmetric dimethylarginine by inducing dimethylarginine dimethylaminohydrolase in rats. Hypertens Res. 2005 Mar; 28 (3): 255-62. PMID: 16097370 5. Chen Y, et al. Dimethylarginine dimethylaminohydrolase and endothelial dysfunction in failing hearts. Am J Physiol Heart Circ Physiol. 2005 Nov; 289(5): H2212-9. PMID: 16024577