Technical Data
DCAF6 (DDB1- and CUL4-associated Factor 6, Androgen Receptor Complex-associated Protein, ARCAP, IQ Motif and WD Repeat-containing Protein 1, Nuclear Receptor Interaction Protein, NRIP, IQWD1, MSTP055)
IQ motif and WD repeats 1 (IQWD1) WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies and their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860aa nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up regulates their transcriptional activity, thereby functioning as a nuclear receptor coactivator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer thus indicating a therapeutic intervention. Northern Blot analysis detected a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
50ug-20CBlue IceHumanRabbit
A portion of aa800-850 of human NRIP.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human NRIP. Species sequence homology: Canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.