		Technical Data
D1660-40G	DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, HLR1, HELR, G17P1, DDX5)

Description:
DDX5, otherwise known as p68 RNA helicase, a nuclear protein and member of the DEAD box protein family which exhibit RNA-dependent ATPase activity, and play important roles in RNA metabolism/ function, including pre-mRNA splicing, RNA translation, RNA synthesis and the assembly of RNP complexes. DDX5 interacts with Smad3 and p53 to modulate transcription, and acts as a transcriptional coactivator for estrogen receptor alpha (ERalpha).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Hela lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to CPMIGYPMPTGYSQ, from the C-terminus of the protein sequence according to NP_004387.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human DDX5. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Iggo R, Gough A, Xu W, Lane DP, Spurr NK. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci USA. 1989 Aug;86(16):6211-4. PMID: 2762324.