Technical Data
D2675-02
Deleted in Azoospermia-Like (DAZL, DAZL1, DAZ Homolog, DAZH, DAZ-like Autosomal, DAZLA, MGC26406, SPGY-like-autosomal, SPGYLA)
Description:
Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (parrafin): Does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase prior to staining. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Histology Positive Control Tissue: Ovary or testis.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from T/O outbred mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B2706Supernatant
SizeStorageShippingSourceHost
2ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to sequence within the C-terminal domain of human DAZL (CRVHHFRRSRAMLKSV). In adult germ cells, the expression of DAZL is localized to the cytoplasm.
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.