Technical Data
D2675-02E
DAZL (Deleted in Azoospermia-like, DAZ-like Autosomal, Deleted in Azoospermia-like 1, DAZ Homolog, SPGY-like-autosomal, DAZL, DAZH, DAZL1, DAZLA, SPGYLA)
Description:
The DAZ (Deleted in AZoospermia) family are potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. DAZL is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.1-0.3ug/ml, observed in human ovary testes lysates on ~35kD bands
Immunohistochemistry (Paraffin): 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-GNGPQKKSVDR, from DAZL, at the internal region of the protein (NP_001342.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human DAZL. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod. 2005 Jun;11(6):405-411. Epub 2005 May 6.