Technical Data
D2728
Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor)
Description:
Delta 4 (Delta 4 Precursor, Delta Ligand 4 Precursor, Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a
transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:25,000
Immunohistochemistry (FFPE): 10-20ug/ml; Detects Delta 4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reports, no staining was observed in vascular tissue.
Western Blot: 1:500-1:2500. Recognizes a band ~74kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to an internal region of human Delta 4.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Delta 4. Species Sequence Homology: mouse, rat and chimpanzee (91%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lauret, E., et al., Leukemia 18(4): 788-797 (2004). 2. Tohda, S., et al., Int. J. Oncol. 22(5): 1073-1079 (2003). 3. Liu, Z.J., et al., Mol. Cell. Biol. 23(1): 14-25 (2003). 4. Dorsch, M., et al., Blood 100(6): 2046-2055 (2002). 5. Mailhos, C., et al., Differentiation 69(2-3): 135-144 (2001). 6. Shutter, J.R., et al., Genes Dev. 14(11): 1313-1318 (2000).