		Technical Data
D2728	Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor)

Description:
Delta 4 (Delta 4 Precursor, Delta Ligand 4 Precursor, Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a
transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:25,000
Immunohistochemistry (FFPE): 10-20ug/ml; Detects Delta 4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reports, no staining was observed in vascular tissue.
Western Blot: 1:500-1:2500. Recognizes a band ~74kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to an internal region of human Delta 4.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

Specificity:

Recognizes human Delta 4. Species Sequence Homology: mouse, rat and chimpanzee (91%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lauret, E., et al., Leukemia 18(4): 788-797 (2004). 2. Tohda, S., et al., Int. J. Oncol. 22(5): 1073-1079 (2003). 3. Liu, Z.J., et al., Mol. Cell. Biol. 23(1): 14-25 (2003). 4. Dorsch, M., et al., Blood 100(6): 2046-2055 (2002). 5. Mailhos, C., et al., Differentiation 69(2-3): 135-144 (2001). 6. Shutter, J.R., et al., Genes Dev. 14(11): 1313-1318 (2000).