Technical Data
D2728-01
Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor)
Description:
Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the -secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). DLL4 expression is highly restricted to the vascular endothelium (7), and haploinsufficiency of DLL4 results in major defects in vascular systems in mouse (8-11). Blockade of DLL4 inhibits tumor growth in model systems (12-14).

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to a region surrounding residue Leu617 of human Delta-like 4 (DLL4) (KLH).
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human DLL4 protein at ~80kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 28602868. 2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320328. 3. Chiba, S. (2006) Stem Cells 24, 24372447. 4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 136013610. 5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 76387643