2’-Deoxyuridine 5’-Triphosphate, Sodium Salt (dUTP) Solution
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Tested in PCR with Taq DNA. Production of 1000bp PCR fragment from 2ng genomic DNA.
Suitable for use in PCR, RT-PCR, cDNA, synthesis and primer extension. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Extinction Coefficient: 10x10e3 (pH 7.0)
Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Endo- and Exonucleases:
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 1ul of 20mM dUTP for 4 hours at 37°C, separation on denaturing polyacrylamide gel and phosphoimaging did not detect DNA degradation.
Incubation of 2000 base RNA transcript with 1ul of 20mM dUTP at 37°C for 4 hours and separation on agarose gel resulted in no decrease in RNA transcript band intensity compared to control.
Incubation of 1ug of supercoiled pUC19 DNA with 1ul of 20mM dUTP at 37°C for 17 hours and separation on agarose gel did not generate linearised plasmid, and relaxation of supercoiled plasmid as compared to control.
Quantitative PCR test on ABI Prism 7000 SDS, which uses amplification of E.coli 23S rRNA gene fragment did not detect E.coli DNA.
Quantitative PCR test on ABI Prism 7000 SDS, which uses amplification of human genomic DNA fragment did not detect human DNA.
PCR amplification of a 354 bp fragment of human apoprotein gene from 50pg human genomic DNA using Hot Start Taq DNA Polymerase.
Purity: 99% (HPLC)
Concentration: 100mM (25umole/250ul)
Form: Supplied as a liquid in 100mM aqueous solution, pH 7.0.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.