Technical Data
DEP1 (1024-1306), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (Density-enhanced Phosphatase-1, PTP-eta, CD148)
Molecular Biology Storage: -70CShipping: Dry Ice
Catalytic domain, (a.a. 1024-1306), MW=32.9kD, expressed in an E. coli expression system.

Stability: 6 months at 20C

Purity: >95% by SDS-PAGE.

Specific Activity: 114 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30C.

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
Source: Recombinant, Human from E. coli
Form: 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 3mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12.
2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.