Suitable for use in Immunofluorescence, Immunoblot. Other applications not tested.
Immunofluorescence (Indirect): 1:10-1:20 (formalin fixed paraffin-embedded sections of human intestine sections). Note: Recommend antigen retrieval with citrate buffer
Optimal dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Purified desmin from chicken gizzard, isolated by modified procedure of Geisler, et al. (J. Biol. Chem. 111: 425-433 (1980)).|
|Supplied as a liquid, serum, 0.1% sodium azide.|
|Reacts specifically with desmin in cultured cells or tissue preparations originating from chicken, human, bovine and mouse tissue. Specifically stains the wide desmin band in immunoblot at a MW of 50–55kD. Species Crossreactivity: Human, bovine, mouse and chicken desmin.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Lazarides, E., Nature 283: 249-256 (1980). 2. Lazarides, E., Ann. Rev. Biochem. 51: 219-250 (1982). 3. Hubbard, B., et al., J. Cell Bio. 80: 166-182 (1979). 4. Bignami, A., et al., J. Histochem. and Cytochem. 32: 473-476 (1984). 5. Lazarides, E., et al., PNAS USA 73: 4344-4348 (1976). 6. Askanas, V., et al., Neurology 40: 949-953 (1990).|