		Technical Data
D3221-02	Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Description:
Applications:
Suitable for use in Immunofluorescence, Immunoblot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (Indirect): 1:10-1:20 (formalin fixed paraffin-embedded sections of human intestine sections). Note: Recommend antigen retrieval with citrate buffer
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Chicken	Rabbit

Concentration:

Not determined

Immunogen:

Purified desmin from chicken gizzard, isolated by modified procedure of Geisler, et al. (J. Biol. Chem. 111: 425-433 (1980)).

Purity:

Serum

Form

Supplied as a liquid, serum, 0.1% sodium azide.

Specificity:

Reacts specifically with desmin in cultured cells or tissue preparations originating from chicken, human, bovine and mouse tissue. Specifically stains the wide desmin band in immunoblot at a MW of 50–55kD. Species Crossreactivity: Human, bovine, mouse and chicken desmin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lazarides, E., Nature 283: 249-256 (1980). 2. Lazarides, E., Ann. Rev. Biochem. 51: 219-250 (1982). 3. Hubbard, B., et al., J. Cell Bio. 80: 166-182 (1979). 4. Bignami, A., et al., J. Histochem. and Cytochem. 32: 473-476 (1984). 5. Lazarides, E., et al., PNAS USA 73: 4344-4348 (1976). 6. Askanas, V., et al., Neurology 40: 949-953 (1990).