Technical Data
D3221-03A
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin, a protein of 53kD, is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomysarcomas (smooth muscle) as well as rhabdomysarcomas (striated muscle).

Applications:
Suitable for use in Immunohistochemistry (Frozen). Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A402Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Immunogen:
Purified Human desmin.
Purity:
Tissue Culture Supernatant
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.09% sodium azide.
Specificity:
Reacts with human desmin in skeletal, cardiac and smooth muscle cells. The antibody recognizes tumors of myogenic origin such as leiomyosarcomas and rhabdomyosarcomas. Species Cross Reactivity: Reported to crossreact with ovine (1), feline (2), rat, chicken, hamster, mouse (3) and human desmin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, Y. Y. and Bailey, R. R. (1998). Treatment of vesicoureteric reflex in a sheep model using subureteric injection of cultured fetal-bladder tissue. Pediatr. Surg. Int. 13: 32-36. 2. Joling, P. et al. (1996). Immunohistochemical demonstration of cellular antigen of the cat defined by anti-human antibodies. Vet. Immunol. Immunopathol. 53: 115-127.

3. Thorey, I.S. et al. (2004). Transgenic mice reveal novel activities of growth hormone in wand repair, angiogenesis and myofibroblast differentiation. J. Biol. Chem. 279(25):26674-26684.