Technical Data
D3221-04
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomysarcomas (smooth muscle) as well as rhabdomysarcomas (striated muscle).

Applications:
Suitable for use in Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 5ug/ml
Immunohistochemistry: 5ug/ml
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1129Affinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified desmin
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes human Desmin from skeletal, cardiac, visceral and some vascular smooth muscle cells. Cell lines (such as RD (ATCC CCL 136) and hamster BHK-21) are positive. Species Crossreactivity: porcine, rat and toad. Species Sequence Homology: mouse, 98%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Debus, E., Weber, K., Osborn, M., EMBO J. 2: 2305-2312 (1983). 2. Altmannsberger, M., et al., Lab. Invest. 46: 520-526 (1982).