Technical Data
Media
Dextrose Phosphate Broth (Powder)
D3222-10
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone10.0
Yeast Extract2.0
Sodium Chloride5.0
Sodium Bisulfite 0.1
Peptic Digest of Animal Tissue10.0
Dextrose1.0
Disodum Phosphate3.0
Sodium Citrate1.0
Total:32g/liter
A standard methods medium for dairy products testing.
Dextrose Phosphate Broth is a modification of yeast autolysate agar. The addition of dibasic sodium phosphate is used to control hydrogen ion concentration which is produced during the utilization of dextrose. Low pH is inhibitory to Brucella.


Directions per Liter: Dissolve 32 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder

Solubility: Light amber, clear to turbid

pH: 7.40.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.