Technical Data
Media
Dextrose Tryptone Broth (Powder)
D3222-25
Microbiological Grade
Storage 4C    Shipping RT
Components shown as g/liter
Tryptone20.0
Dextrose10.0
Bromcresol Purple 0.04
Total:30.04g/liter
Description:
Dextrose Tryptone Broth is a non-selective medium which can support the growth of a variety of mesophilic and thermophilic microorganisms. It contains the pH indicator bromcresol purple. Inoculated filter membranes are incubated on this medium at 55C to detect and enumerate thermophilic "flat sour" sporulating microorganisms. Isolation and enumeration of flat-sour thermophiles and mesophiles in food by membrane filter technique.


Directions per Liter: Suspend 30g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Grayish-beige, homogeneous, free flowing powder.

Solubility: Purple, clear, complete.

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.