Technical Data
DGAT1 (Diacylglycerol O-acyltransferase 1, EC, Diglyceride Acyltransferase, ACT-related Gene Product 1)
Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Cellular Localization: Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protein.

Positive Control: HepG2 lysate

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: A band is seen at ~55kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide within an internal region (residues 200-300) of the human DGAT1 protein. [Swiss-Prot# O75907]. Homology: 100% with human, mouse, bovine, Zebrafish, and rat.
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Species Crossreactivity: This antibody reacts with the human protein. Other species have not been determined.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.