		Technical Data
D3790-01H	DJ-1 (PARK7, Protein DJ-1, Oncogene DJ1, Parkinson Disease Protein 7)

Description:
Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Mutations in a-synuclein, Parkin and DJ-1 have linked these genes to PD. Alpha-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice resulting in reduced long-term depression (6).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	D21E11	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues surrounding Gly150 of human DJ-1.

Purity:

Supernatant

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes endogenous levels of total human DJ-1. It may also detect mouse and rat samples weakly.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Borrelli, E. (2005) Neuron 45, 479-81. 2.Bonifati, V. et al. (2003) Science 299, 256-9. 3.Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13. 4.Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63. 5.Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93. 6.Goldberg, M.S. et al. (2005) Neuron 45, 489-96.