Technical Data
D3810
Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)
Description:
The human Dickkopf (Dkk)-related proteins are a structurally and functionally heterogeneous family of proteins. This family is comprised of hDkk1, -2, -3, -4 and Soggy (Sgy), a Dkk-3 related protein. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues and are secreted when expressed in 293T cells. Several secreted antagonists, including Dickkopf-1 (Dkk-1) (German for “big head, stubborn”) regulate the activity of Wnt proteins secreted glycoproteins that influence many aspects of embryonic development in metazoans. Dkk-1 is a head inducer secreted from the vertebrate head organizer, that induces anterior development of Xenopus by antagonizing Wnt signaling. Dkk-1 inhibits Wnt-induced axis duplication, acts upstream of disheveled3 and antagonizes Wnt signaling upstream of its effect on beta-catenin regulation. Dkk-1 is an LRP6 ligand5 that inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation. Dkk-1 plays a crucial role in the control of programmed cell death during vertebrate limb development. Bmp-4 is one of several upstream regulators of Dkk-1 expression. Furthermore, the proapoptotic hDkk-1 gene may play important roles in linking the oncogenic Wnt and p53 tumor suppressor pathways.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
0.125mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal region of the human and mouse Dkk-1(Dickkopf-1) proteins.
Purity:
Purified by epitope affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human and mouse Dkk-1 proteins. On Western blots, it identifies the target band at ~35kD. Reactivity has been confirmed with human Dkk-1-transfected 293T cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krupnik VE, et al. Gene 238(2):301-313, 1999.2. Niehrs C, et al. Int J Dev Biol 45(1):237-240, 2001.3. Glinka A, et al. Nature 391(6665):357-362, 1998.4. Fedi P, et al. J Biol Chem 274(27):19465-19472, 1999.5. Balfico A, et al. Nat Cell Biol 3(7):683-686, 2001.6. Semenov MV, et alCurr Biol 11(12):951-961, 2001.7. Grotewold L & Ruther U. Int J Dev Biol 46(7):943-947, 2002a.8. Grotewold L & Ruther U. EMBO J 21(5):966-975, 2002b.9. Shou J, et al. Oncogene 21(6):878-889, 2002.