Technical Data
D3810-01A
Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)
Description:
Dickkopf (DKK) family of proteins consist of four members DKK1, DKK2, DKK3 and DKK4 and function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (810). Increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Gln75 of human DKK1.
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Mao, B. et al. (2001) Nature 411, 3215.
(2) Niehrs, C. (2006) Oncogene 25, 746981.
(3) Krupnik, V.E. et al. (1999) Gene 238, 30113.
(4) Monaghan, A.P. et al. (1999) Mech Dev 87, 4556.
(5) Mao, B. et al. (2002) Nature 417, 6647.
(6) Davidson, G. et al. (2002) Development 129, 558796.
(7) Glinka, A. et al. (1998) Nature 391, 35762.
(8) Baron, R. and Rawadi, G. (2007) Curr Osteoporos Re 5, 7380.
(9) Macdonald, B.T. et al. (2007) Bone, epub ahead of print.
(10) Diarra, D. et al. (2007) Nat Med 13, 15663.
(11) Forget, M.A. et al. (2007) Br J Cancer 96, 64653.
(12) Yamabuki, T. et al. (2007) Cancer Res 67, 251725.
(13) Kuphal, S. et al. (2006) Oncogene 25, 502736.
(14) Aguilera, O. et al. (2006) Oncogene 25, 411621.