Technical Data
D3931-61G
DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)
Description:
DNMT1 is a nuclear enzyme with a major role in the maintenance of DNA methylation patterns after replication. DNA methylation is significant for epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development. Overexpression of DNMT1 has been detected in several human cancers.

Blocking Peptide: D3931-61T

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5001: 5,000
Immunoprecipitation: 2-4 ug/mg protein
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
Sheep anti rabbit IgG: HRP

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a region within human DNMT1 encoded within exon 25.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human DNA (cytosine-5) methyltransferase 1, also known as DNMT1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.